Share Email Print
cover

Proceedings Paper

Optofluidic control of axonal guidance
Author(s): Ling Gu; Simon Ordonez; Bryan Black; Samarendra K. Mohanty
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Significant efforts are being made for control on axonal guidance due to its importance in nerve regeneration and in the formation of functional neuronal circuitry in-vitro. These include several physical (topographic modification, optical force, and electric field), chemical (surface functionalization cues) and hybrid (electro-chemical, photochemical etc) methods. Here, we report comparison of the effect of linear flow versus microfluidic flow produced by an opticallydriven micromotor in guiding retinal ganglion axons. A circularly polarized laser tweezers was used to hold, position and spin birefringent calcite particle near growth cone, which in turn resulted in microfluidic flow. The flow rate and resulting shear-force on axons could be controlled by a varying the power of the laser tweezers beam. The calcite particles were placed separately in one chamber and single particle was transported through microfluidic channel to another chamber containing the retina explant. In presence of flow, the turning of axons was found to strongly correlate with the direction of flow. Turning angle as high as 90° was achieved. Optofluidic-manipulation can be applied to other types of mammalian neurons and also can be extended to stimulate mechano-sensing neurons.

Paper Details

Date Published: 8 March 2013
PDF: 7 pages
Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860T (8 March 2013); doi: 10.1117/12.2007692
Show Author Affiliations
Ling Gu, The Univ. of Texas at Arlington (United States)
Simon Ordonez, The Univ. of Texas at Arlington (United States)
Bryan Black, The Univ. of Texas at Arlington (United States)
Samarendra K. Mohanty, The Univ. of Texas at Arlington (United States)


Published in SPIE Proceedings Vol. 8586:
Optogenetics: Optical Methods for Cellular Control
Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

© SPIE. Terms of Use
Back to Top