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Proceedings Paper

Discriminating model for skin cancer diagnosis in vivo through Raman spectroscopy
Author(s): Fabrício Luiz Silveira; Marcos Tadeu T. Pacheco; Benito Bodanese; Renato Amaro Zângaro; Landulfo Silveira
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Paper Abstract

This work aimed the development of a discriminating model, using Raman spectroscopy, based on the estimated concentration of biochemical components presented in skin, for in vivo diagnosis. Raman spectra were collected in patients who underwent excision surgery of suspicious lesions at the lesion site and at a normal circumjacent site. It has been estimated the relative amount of selected biochemical compounds presented in skin. The Raman spectra of normal and malignant (basal cell carcinoma - BCC and squamous cell carcinoma - SCC) skin are quite similar, with some spectral differences in the regions of lipids, nucleic acids, and hemoglobin. Some biochemicals showed statistically significant differences among N, BCC and SCC, such as elastin, ceramide, melanin, nucleid acid, actin and phenylalanine. Elastin and ceramide presented significant differences between N and BCC, melanin, DNA and actin presented significant differences between N and BCC and between N and SCC, being melanin and DNA decreased in neoplasias, in contrast with actin, that increased in neoplasias. Concentration of phenylalanine was significantly increased for SCC compared to N and BCC. The relative concentration of melanin, DNA and phenylalanine showed sensitivity, specificity and accuracy of about 81%, 65% and 60%, respectively, using Mahalanobis distance as a discriminator. This model is being incorporated to a Raman system with automated data collection and processing that could be used for a future in vivo, real time discrimination algorithm.

Paper Details

Date Published: 8 March 2013
PDF: 8 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85650Y (8 March 2013); doi: 10.1117/12.2007293
Show Author Affiliations
Fabrício Luiz Silveira, Univ. Camilo Castelo Branco (Brazil)
Marcos Tadeu T. Pacheco, Univ. Camilo Castelo Branco (Brazil)
Benito Bodanese, Univ. Comunitária Regional de Chapecó (Brazil)
Renato Amaro Zângaro, Univ. Camilo Castelo Branco (Brazil)
Landulfo Silveira, Univ. Camilo Castelo Branco (Brazil)


Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory; Guillermo J. Tearney; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen; E. Duco Jansen; Justus F. Ilgner; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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