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Proceedings Paper

A new expanded point information content design approach for 3D live-cell microscopy at video rates
Author(s): Ramzi N. Zahreddine; Robert H. Cormack; Carol J. Cogswell
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Paper Abstract

Through a combination of optical design and algorithm development, a new expanded point information content (EPIC) microscope has been developed that is capable of extending the depth of field while simultaneously super locating the depth position of complex biological objects to within an accuracy of 75 nm. The data is then combined to form 3D animations of live-cell biological specimens. This is accomplished without the need to acquire multi-focal image stacks and is thus well suited for high-speed imaging.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858902 (22 February 2013); doi: 10.1117/12.2006493
Show Author Affiliations
Ramzi N. Zahreddine, Univ. of Colorado Boulder (United States)
Robert H. Cormack, Univ. of Colorado Boulder (United States)
Carol J. Cogswell, Univ. of Colorado Boulder (United States)


Published in SPIE Proceedings Vol. 8589:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson, Editor(s)

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