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Proceedings Paper

Biodistribution study of nanoparticle encapsulated photodynamic therapy drugs using multispectral imaging
Author(s): Luma V. Halig; Dongsheng Wang; Andrew Y. Wang; Zhuo Georgia Chen; Baowei Fei
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Paper Abstract

Photodynamictherapy (PDT) uses a drug called a photosensitizer that is excited by irradiation with a laser light of a particular wavelength, which generates reactive singlet oxygen that damages the tumor cells. The photosensitizer and light are inert; therefore, systemic toxicities are minimized in PDT. The synthesis of novel PDT drugs and the use of nanosized carriers for photosensitizers may improve the efficiency of the therapy and the delivery of the drug. In this study, we formulated two nanoparticles with and without a targeting ligand to encapsulate phthalocyanines 4 (Pc 4) molecule and compared their biodistributions. Metastatic human head and neck cancer cells (M4e) were transplanted into nude mice. After 2-3 weeks, the mice were injected with Pc 4, Pc 4 encapsulated into surface coated iron oxide (IO-Pc 4), and IO-Pc 4 conjugated with a fibronectin-mimetic peptide (FMP-IO-Pc 4) which binds specifically to integrin β1. The mice were imaged using a multispectral camera. Using multispectral images, a library of spectral signatures was created and the signal per pixel of each tumor was calculated, in a grayscale representation of the unmixed signal of each drug. An enhanced biodistribution of nanoparticle encapsulated PDT drugs compared to non-formulated Pc 4 was observed. Furthermore, specific targeted nanoparticles encapsulated Pc 4 has a quicker delivery time and accumulation in tumor tissue than the non-targeted nanoparticles. The nanoparticle-encapsulated PDT drug can have a variety of potential applications in cancer imaging and treatment.

Paper Details

Date Published: 29 March 2013
PDF: 8 pages
Proc. SPIE 8672, Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging, 867218 (29 March 2013); doi: 10.1117/12.2006492
Show Author Affiliations
Luma V. Halig, Emory Univ. (United States)
Dongsheng Wang, Emory Univ. (United States)
Andrew Y. Wang, Ocean NanoTech LLC (United States)
Zhuo Georgia Chen, Emory Univ. (United States)
Baowei Fei, Emory Univ. (United States)
Emory Univ. and Georgia Institute of Technology (United States)

Published in SPIE Proceedings Vol. 8672:
Medical Imaging 2013: Biomedical Applications in Molecular, Structural, and Functional Imaging
John B. Weaver; Robert C. Molthen, Editor(s)

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