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Proceedings Paper

Quantitative segmentation of fluorescence microscopy images of heterogeneous tissue: Approach for tuning algorithm parameters
Author(s): Jenna L. Mueller; Zachary T. Harmany; Jeffrey K. Mito; Stephanie A. Kennedy; Yongbaek Kim; Leslie Dodd; Joseph Geradts; David G. Kirsch; Rebecca M. Willett; J. Quincy Brown; Nimmi Ramanujam
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Paper Abstract

The combination of fluorescent contrast agents with microscopy is a powerful technique to obtain real time images of tissue histology without the need for fixing, sectioning, and staining. The potential of this technology lies in the identification of robust methods for image segmentation and quantitation, particularly in heterogeneous tissues. Our solution is to apply sparse decomposition (SD) to monochrome images of fluorescently-stained microanatomy to segment and quantify distinct tissue types. The clinical utility of our approach is demonstrated by imaging excised margins in a cohort of mice after surgical resection of a sarcoma. Representative images of excised margins were used to optimize the formulation of SD and tune parameters associated with the algorithm. Our results demonstrate that SD is a robust solution that can advance vital fluorescence microscopy as a clinically significant technology.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871F (22 February 2013); doi: 10.1117/12.2006429
Show Author Affiliations
Jenna L. Mueller, Duke Univ. (United States)
Zachary T. Harmany, The Univ. of Wisconsin-Madison (United States)
Jeffrey K. Mito, Duke Univ. School of Medicine (United States)
Stephanie A. Kennedy, Duke Univ. (United States)
Yongbaek Kim, Seoul National Univ. (Korea, Republic of)
Leslie Dodd, Univ. of North Carolina School of Medicine (United States)
Joseph Geradts, Duke Univ. Medical Ctr. (United States)
David G. Kirsch, Duke Univ. School of Medicine (United States)
Rebecca M. Willett, Duke Univ. (United States)
J. Quincy Brown, Tulane Univ. (United States)
Nimmi Ramanujam, Duke Univ. (United States)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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