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Proceedings Paper

Reflectance spectroscopy for evaluating optical clearing efficacy of skin in vivo
Author(s): Xiewei Zhong; Jing Wang; Rui Shi; Dan Zhu
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Paper Abstract

Chemical agents with high refractive index, hyperosmotic, and biocompatibility are introduced into tissue, which will reduce the scattering of tissue, and enhance the penetration of light in tissue. Diffuse reflectance, as a common method, has been applied to assess optical clearing of skin in vivo, but the scattering characteristic during the in-vivo optical clearing process has not been valuated quantitatively. In this work, a diffuse reflectance spectroscopy, based on a lookuptable (LUT) based inverse model, is applied to calculate the reduced scattering coefficient and absorption coefficient of skin. Optical clearing agents (OCAs) were topically treated on mouse skin in vivo. The diffuse reflectance during optical clearing was recorded, and the optical properties can be extracted by the reflectance spectroscopy. The results show that the diffuse reflectance spectra and the reduced scattering coefficient are decreased obviously, whilst the absorption coefficient is increased after the application of OCAs. This study provides evident directly for explore the mechanisms of optical clearing of skin in vivo.

Paper Details

Date Published: 1 March 2013
PDF: 6 pages
Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 85800P (1 March 2013); doi: 10.1117/12.2006011
Show Author Affiliations
Xiewei Zhong, Huazhong Univ. of Science and Technology (China)
Jing Wang, Huazhong Univ. of Science and Technology (China)
Rui Shi, Huazhong Univ. of Science and Technology (China)
Dan Zhu, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 8580:
Dynamics and Fluctuations in Biomedical Photonics X
Valery V. Tuchin; Donald D. Duncan; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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