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Proceedings Paper

In vivo reactive neural plasticity investigation by means of correlative two photon: electron microscopy
Author(s): A. L. Allegra Mascaro; P. Cesare; L. Sacconi; G. Grasselli; G. Mandolesi; B. Maco; G. Knott; L. Huang; V. De Paola; P. Strata; F. S. Pavone
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Paper Abstract

In the adult nervous system, different populations of neurons correspond to different regenerative behavior. Although previous works showed that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury1, we have hitherto no details about the real dynamics of fiber regeneration. We set up a model of singularly axotomized climbing fibers (CF) to investigate their reparative properties in the adult central nervous system (CNS) in vivo. Time lapse two-photon imaging has been combined to laser nanosurgery2, 3 to define a temporal pattern of the degenerative event and to follow the structural rearrangement after injury. To characterize the damage and to elucidate the possible formation of new synaptic contacts on the sprouted branches of the lesioned CF, we combined two-photon in vivo imaging with block face scanning electron microscopy (FIB-SEM). Here we describe the approach followed to characterize the reactive plasticity after injury.

Paper Details

Date Published: 22 February 2013
PDF: 9 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881A (22 February 2013); doi: 10.1117/12.2005953
Show Author Affiliations
A. L. Allegra Mascaro, Univ. of Florence (Italy)
P. Cesare, Univ. of Turin (Italy)
EBRI-Santa Lucia Foundation (IRCCS) (Italy)
L. Sacconi, National Institute of Optics-National Research Council (Italy)
INO-Consiglio Nazionale delle Ricerche (Italy)
G. Grasselli, Fondazione Santa Lucia (Italy)
G. Mandolesi, Fondazione Santa Lucia (Italy)
B. Maco, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
G. Knott, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
L. Huang, Imperial College London (United Kingdom)
V. De Paola, Imperial College London (United Kingdom)
P. Strata, Univ. of Turin (Italy)
Fondazione Santa Lucia (Italy)
F. S. Pavone, Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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