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Proceedings Paper

Enhanced delivery of gold nanoparticles by acoustic cavitation for photoacoustic imaging and photothermal therapy
Author(s): Yu-Hsin Wang; Ai-Ho Liao; Jia-Yu Lin; Cheng-Ru Lee; Cheng-Ham Wu; Tzu-Min Liu; Churng-Ren Wang; Pai-Chi Li
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Paper Abstract

Gold-nanorods incorporated with microbubbles (AuMBs) were introduced as a photoacoustic/ultrasound dual- modality contrast agent in our previous study. The application can be extended to theragnosis purpose. With the unique physical characteristics of AuMBs, we propose an enhanced delivery method for the encapsulated particles. For example, laser thermotherapy mediated by plasmonic nanoparticles can be made more effective by using microbubbles as a targeted carrier and acoustic cavitation for enhanced sonoporation. The hypothesis was experimentally tested. Firts, these AuMBs first act as molecular probes with binding to specific ligands. The improved targeting efficacy was macroscopically observed by an ultrasound system. The extended retention of targeted AuMB was observed and recorded for 30 minutes in a CT-26 tumor bearing mouse. Secondly, cavitation induced by time-varying acoustic field was also applied to disrupt the microbubbles and cause increased transient cellular permeability (a.k.a., sonoporation). Multimodal optical microscope based on a Cr:forsterite laser was used to directly observe these effects. The microscope can acquired third-harmonic generation (THG) and two-photon fluorescent (2PF) signals produced by the AuMBs. In vitro examination shows approximately a 60% improvement in terms of fluorescence signals from the cellular uptake of gold nanoparticles after sonoporation treatment. Therefore, we conclude that the controlled release is feasible and can further improve the therapeutic effects of the nanoparticles.

Paper Details

Date Published: 4 March 2013
PDF: 8 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858123 (4 March 2013); doi: 10.1117/12.2005870
Show Author Affiliations
Yu-Hsin Wang, National Taiwan Univ. (Taiwan)
Ai-Ho Liao, National Taiwan Univ. of Science and Technology (Taiwan)
Jia-Yu Lin, National Chung-Cheng Univ. (Taiwan)
Cheng-Ru Lee, National Chung-Cheng Univ. (Taiwan)
Cheng-Ham Wu, National Taiwan Univ. (Taiwan)
Tzu-Min Liu, National Taiwan Univ. (Taiwan)
Churng-Ren Wang, National Chung Cheng Univ. (Taiwan)
Pai-Chi Li, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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