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Proceedings Paper

Integrated liquid jet waveguide for fluorescence spectroscopy on chip
Author(s): Gianluca Persichetti; Genni Testa; Romeo Bernini
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Paper Abstract

An optofluidic jet waveguide for on chip fluorescence analysis is presented. The waveguide consists of an high speed water jet produced by means of a micro-channel coupled with a multimode optical fiber collecting the fluorescence opportunely excited. The liquid jet acts, at the same time, as the solution to analyse and as an optical waveguide. This configuration allows a strong reduction of the scattering and fluorescence of non analyte substances enabling a very low limit of detection (LOD). The integrated device is fabricated by PMMA micro-machining allowing a self-alignment between the liquid jet waveguide and the optical fiber used to deliver the fluorescence to the detector. The performance of the system has been tested on Cy5 water solutions and LOD of 2.56 nM has been obtained. A proof-of-concept of filter-free measurements has been performed demonstrating that fluorescence measurements can be performed also by using a photodiode with an LOD of 6.11 nM.

Paper Details

Date Published: 9 March 2013
PDF: 6 pages
Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 861502 (9 March 2013); doi: 10.1117/12.2005837
Show Author Affiliations
Gianluca Persichetti, Institute for Electromagnetic Monitoring of the Environment, CNR (Italy)
Genni Testa, Institute for Electromagnetic Monitoring of the Environment, CNR (Italy)
Romeo Bernini, Institute for Electromagnetic Monitoring of the Environment, CNR (Italy)


Published in SPIE Proceedings Vol. 8615:
Microfluidics, BioMEMS, and Medical Microsystems XI
Holger Becker; Bonnie L. Gray, Editor(s)

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