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Proceedings Paper

New approach to double-helix point spread function design for 3D super-resolution microscopy
Author(s): Ginni Grover; Rafael Piestun
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Paper Abstract

Double-helix point spread functions (DH-PSFs) have been used to extend localization and super-resolution microscopy to three dimensions. Current DH-PSF design provides a long depth-of-field for 3D imaging. However, it is not optimal for imaging under high background conditions. We present a method to design unconventional DH-PSFs with control on characteristics such as efficiency, transverse spread, and Fisher information. This allows tailoring the PSFs for specific applications. In particular, we introduce a design suitable for 3D localization-based super-resolution imaging under typical background conditions and demonstrate imaging with resolution one order of magnitude beyond the diffraction limit over a depth-of-field of 1.2 μm.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900M (22 February 2013); doi: 10.1117/12.2005694
Show Author Affiliations
Ginni Grover, Univ. of Colorado Boulder (United States)
Rafael Piestun, Univ. of Colorado Boulder (United States)

Published in SPIE Proceedings Vol. 8590:
Single Molecule Spectroscopy and Superresolution Imaging VI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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