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Proceedings Paper

Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy
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Paper Abstract

By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Paper Details

Date Published: 4 March 2013
PDF: 6 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858127 (4 March 2013); doi: 10.1117/12.2005669
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Chih-Hsien Huang, Texas A&M Univ. (United States)
Catherine Martel, Washington Univ. School of Medicine (United States)
Konstantin I. Maslov, Washington Univ. in St. Louis (United States)
Lidai Wang, Washington Univ. in St. Louis (United States)
Joon-Mo Yang, Washington Univ. in St. Louis (United States)
Liang Gao, Washington Univ. in St. Louis (United States)
Gwendalyn Randolph, Washington Univ. School of Medicine (United States)
Jun Zou, Texas A&M Univ. (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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