Share Email Print
cover

Proceedings Paper

Optical spectroscopy methods to probe key spectral fingerprints of animal bone
Author(s): Diana C. Sordillo; Laura A. Sordillo; Peter P. Sordillo; Robert Alfano
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The chemical composition of different animal bones was studied using Raman spectroscopy and fluorescence spectroscopy. Using a luminescence spectrometer, the fluorescence spectra from an excitation of 300 nm, 340 nm, 400 nm and 500 nm were investigated and showed the key molecular components of bone. After 300 nm excitation, 380 nm fluorescence was seen, consistent with the presence of collagen. After 340 nm excitation, 380 nm fluorescence was seen, consistent with the presence of collagen, the main organic material in bone. An excitation of 340 nm also showed a fluorescence of 450 nm, most likely due to the presence of NADH. After 500 nm excitation, 600 nm fluorescence was seen, most likely caused by porphyrins due to blood within the bone. Raman spectroscopy using a 785 nm laser was also performed. With these spectroscopic tools, the important features and components of bone can be studied.

Paper Details

Date Published: 8 March 2013
PDF: 6 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 856568 (8 March 2013); doi: 10.1117/12.2005647
Show Author Affiliations
Diana C. Sordillo, The City College of the City Univ. of New York (United States)
Laura A. Sordillo, The City College of the City Univ. of New York (United States)
Peter P. Sordillo, The City College of the City Univ. of New York (United States)
Robert Alfano, The City College of the City Univ. of New York (United States)


Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory; Guillermo J. Tearney; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen; E. Duco Jansen; Justus F. Ilgner; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

© SPIE. Terms of Use
Back to Top