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Proceedings Paper

Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo
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Paper Abstract

To control the overall action of the body, brain consumes a large amount of energy in proportion to its volume. In humans and many other species, the brain gets most of its energy from oxygen-dependent metabolism of glucose. An abnormal metabolic rate of glucose and/or oxygen usually reflects a diseased status of brain, such as cancer or Alzheimer’s disease. We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood-brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively unmixed by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. The glucose response amplitude was about half that of the hemodynamic response. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area showed a clear vascular pattern and spread about twice as wide as that of the glucose response. The PACT of mouse brain metabolism was validated by high-resolution open-scalp OR-PAM and fluorescence imaging. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.

Paper Details

Date Published: 4 March 2013
PDF: 6 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85814A (4 March 2013); doi: 10.1117/12.2005645
Show Author Affiliations
Junjie Yao, Washington Univ. in St. Louis (United States)
Jun Xia, Washington Univ. in St. Louis (United States)
Konstantin Maslov, Washington Univ. in St. Louis (United States)
Mohammadreza R. N. Avanaki, Washington Univ. in St. Louis (United States)
Vassiliy Tsytsarev, Univ. of Maryland School of Medicine (United States)
Alexei V. Demchenko, Univ. of Missouri-St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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