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Proceedings Paper

Microscale tools for measuring spatiotemporal chemical gradients in biological systems
Author(s): John B. Wydallis; Charles S. Henry; David S. Dandy; Stuart Tobet
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Paper Abstract

Chemical gradients drive many processes in biology, ranging from nerve signal transduction to ovulation. At present, microscopy is the primary tool used to understand these gradients. Microscopy has provided many important breakthroughs in our understanding of the fundamental biology, but is limited due to the need to incorporate fluorescent molecules into a biological system. As a result, there is a need to develop tools that can measure chemical gradient formation in biological systems that do not require fluorescent modification of the molecules in question, can be multiplexed to measure more than one molecule and is compatible with a variety of biological sample types, including in vitro cell cultures and ex vivo tissue slices. Work from our group centered on the development of microscale tools to measure chemical gradients will be presented. In this project, we have developed a microfluidic interface that allows for sampling from underneath a tissue slice or in vitro cell culture system. The sampling system can resolve up to 19 different ports and can be interfaced with either electrochemical or fluorescence-based detection methods. Using these two detection methods, we are capable of analyzing the release of either small molecule metabolites or proteins and peptides using immunoassays.

Paper Details

Date Published: 5 March 2013
PDF: 6 pages
Proc. SPIE 8570, Frontiers in Biological Detection: From Nanosensors to Systems V, 857009 (5 March 2013); doi: 10.1117/12.2005640
Show Author Affiliations
John B. Wydallis, Colorado State Univ., Fort Collins (United States)
Charles S. Henry, Colorado State Univ., Fort Collins (United States)
David S. Dandy, Colorado State Univ., Fort Collins (United States)
Stuart Tobet, Colorado State Univ., Fort Collins (United States)

Published in SPIE Proceedings Vol. 8570:
Frontiers in Biological Detection: From Nanosensors to Systems V
Benjamin L. Miller; Philippe M. Fauchet, Editor(s)

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