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Proceedings Paper

Maximum imaging depth comparison in porcine vocal folds using 776-nm vs. 1552-nm excitation wavelengths
Author(s): Murat Yildirim; Onur Ferhanoglu; James B. Kobler; Steven M. Zeitels; Adela Ben-Yakar
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Paper Abstract

Vocal fold scarring is one of the major causes of voice disorders and may arise from overuse or post-surgical wound healing. One promising treatment utilizes the injection of soft biomaterials aimed at restoring viscoelasticity of the outermost vibratory layer of the vocal fold, superficial lamina propria (SLP). However, the density of the tissue and the required injection pressure impair proper localization of the injected biomaterial in SLP. To enhance treatment effectiveness, we are investigating a technique to image and ablate sub-epithelial planar voids in vocal folds using ultrafast laser pulses to better localize the injected biomaterial. It is challenging to optimize the excitation wavelength to perform imaging and ablation at depths suitable for clinical use. Here, we compare maximum imaging depth using two photon autofluorescence and second harmonic generation with third-harmonic generation imaging modalities for healthy porcine vocal folds. We used a home-built inverted nonlinear scanning microscope together with a high repetition rate (2 MHz) ultrafast fiber laser (Raydiance Inc.). We acquired both two-photon autofluorescence and second harmonic generation signals using 776 nm wavelength and third harmonic generation signals using 1552 nm excitation wavelength. We observed that maximum imaging depth with 776 nm wavelength is significantly improved from 114 μm to 205 μm when third harmonic generation is employed using 1552 nm wavelength, without any observable damage in the tissue.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858825 (22 February 2013); doi: 10.1117/12.2005630
Show Author Affiliations
Murat Yildirim, The Univ. of Texas at Austin (United States)
Onur Ferhanoglu, The Univ. of Texas at Austin (United States)
James B. Kobler, Massachusetts General Hospital (United States)
Steven M. Zeitels, Massachusetts General Hospital (United States)
Adela Ben-Yakar, The Univ. of Texas at Austin (United States)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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