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Proceedings Paper

Polarization-coded coherent anti-Stokes Raman scattering microscopy for nonresonant background suppression
Author(s): Jian Lin; Paul Kumar Upputuri; Gong Li; Haifeng Wang; Zhiwei Huang
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Paper Abstract

The existence of non-resonant background severely decreases the image contrast and may overwhelm the resonant signal from small scatterers in coherent anti-Stokes Raman scattering (CARS) microscopy. In this study, we found that by using circularly polarized pump and Stokes excitations with inverse rotation directions, the non-resonant as well as the resonant CARS generation from molecules with symmetry can be totally suppressed, whereas the resonant CARS signal from asymmetric molecules preserves. This unique property of circularly polarized CARS is useful for high-contrast imaging of asymmetric samples without the interference of non-resonant background, which is demonstrated by the imaging of natural silk fibers.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858809 (22 February 2013); doi: 10.1117/12.2005561
Show Author Affiliations
Jian Lin, National Univ. of Singapore (Singapore)
Paul Kumar Upputuri, National Univ. of Singapore (Singapore)
Gong Li, National Univ. of Singapore (Singapore)
Haifeng Wang, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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