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Proceedings Paper

Photodynamic Antimicrobial Chemotherapy (PACT) Using Phenothiazines Derivatives Associated with the Red Laser against Staphylococcus aureus
Author(s): Susana C. P. S. Oliveira; Gustavo M. P. Santos; Juliana S. C. Monteiro; Anderson F. S. Miranda; Fernando J. P. Sampaio; Maria F. M. Gesteira; Fátima A. A. Zanin; Marcos A. V. Santos; Antônio L. B. Pinheiro
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Paper Abstract

The objective of this study was to evaluate the bactericidal effect of photodynamic antimicrobial chemotherapy (PACT) using phenothiazinium dye (Toluidine blue O and methylene blue) at a low concentration of 1μg/mL irradiated with the red laser at doses of 2.4 e 4.8 J/cm² on strain of Staphylococcus aureus (ATCC 23529) in vitro. For this research, tests were performed in triplicate and the samples were distributed into six test groups: (L-P-) Negative control (L1+P-) and (L2+P-) bacterial suspensions were irradiated with laser energy 2.4 and 4.8 J/cm2 respectively in the absence of photosensitizer; (L1+P+) and (L2+P+) bacterial suspensions were irradiated with laser in the presence of 1μg/ml of photosensitizer and finally (L-P+) bacterial suspensions only in the presence of phenothiazinium dye. Therefore, were analyzed the potential bactericidal PACT by counting of colony-forming units and analyzed statistically (ANOVA, Tukey test, p<0.05). The results showed that the negative control group when compared with laser group (L2+P-) it was observed a statistically significant increase (p<0.01) which L2+P- showed a higher number of CFU, on the other hand when compared to L1+P- no statistically significant difference was found, relation to the groups submitted to PACT, only showed a statistically significant reduction relative to the group irradiated L2+P+ (p<0.01) that showed a decrease in the number of CFU. There was no statistically significant difference between the groups submitted to PDT (L1+P+ and L2+P+). Although the results of this study have shown a reduction in average number of colony forming units by the appropriate laser-dye treatment combination, it needs further investigation.

Paper Details

Date Published: 5 March 2013
PDF: 6 pages
Proc. SPIE 8569, Mechanisms for Low-Light Therapy VIII, 85690P (5 March 2013); doi: 10.1117/12.2005560
Show Author Affiliations
Susana C. P. S. Oliveira, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Gustavo M. P. Santos, Gonçalo Muniz Institute (Brazil)
Juliana S. C. Monteiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Anderson F. S. Miranda, Gonçalo Muniz Institute (Brazil)
Fernando J. P. Sampaio, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Maria F. M. Gesteira, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Fátima A. A. Zanin, Univ. Federal da Bahia (Brazil)
Brugnera & Zanin Institute (Brazil)
Marcos A. V. Santos, Gonçalo Muniz Institute (Brazil)
Antônio L. B. Pinheiro, Univ. Federal da Bahia (Brazil)
National Institute of Optics and Photonics (Brazil)
Ministry of Health, Salvador (Brazil)


Published in SPIE Proceedings Vol. 8569:
Mechanisms for Low-Light Therapy VIII
Michael R. Hamblin; Juanita Anders; James D. Carroll; Praveen R. Arany, Editor(s)

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