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Proceedings Paper

Cell cycle imaging with quantitative differential interference contrast microscopy
Author(s): Piotr Kostyk; Shelley Phelan; Min Xu
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Paper Abstract

We report a microscopic approach for determining cell cycle stages by measuring the nuclear optical path length (OPL) with quantitative differential interference contrast (DIC) microscopy. The approach is validated by the excellent agreement between the proportion of proliferating-to-quiescent cancerous breast epithelial cells obtained from DIC microscopy, and that from a standard immunofluorescence assay.

Paper Details

Date Published: 22 February 2013
PDF: 5 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870J (22 February 2013); doi: 10.1117/12.2005540
Show Author Affiliations
Piotr Kostyk, Fairfield Univ. (United States)
Shelley Phelan, Fairfield Univ. (United States)
Min Xu, Fairfield Univ. (United States)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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