Share Email Print
cover

Proceedings Paper

Investigating real-time activation of adenosine receptors by bioluminescence resonance energy transfer technique
Author(s): Yimei Huang; Hongqin Yang; Liqin Zheng; Jiangxu Chen; Yuhua Wang; Hui Li; Shusen Xie
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Adenosine receptors play important roles in many physiological and pathological processes, for example regulating myocardial oxygen consumption and the release of neurotransmitters. The activations of adenosine receptors have been studied by some kinds of techniques, such as western blot, immunohistochemistry, etc. However, these techniques cannot reveal the dynamical response of adenosine receptors under stimulation. In this paper, bioluminescence resonance energy transfer technique was introduced to study the real-time activation of adenosine receptors by monitoring the dynamics of cyclic adenosine monophosphate (cAMP) level. The results showed that there were significant differences between adenosine receptors on real-time responses under stimulation. Moreover, the dynamics of cAMP level demonstrated that competition between adenosine receptors existed. Taken together, our study indicates that monitoring the dynamics of cAMP level using bioluminescence resonance energy transfer technique could be one potential approach to investigate the mechanism of competitions between adenosine receptors.

Paper Details

Date Published: 21 February 2013
PDF: 6 pages
Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960A (21 February 2013); doi: 10.1117/12.2005507
Show Author Affiliations
Yimei Huang, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Liqin Zheng, Fujian Normal Univ. (China)
Jiangxu Chen, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Hui Li, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 8596:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top