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Proceedings Paper

A fast full-body fluorescence/bioluminescence imaging system for small animals
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Paper Abstract

Whole body in vivo optical imaging of small animals has widened its applications and increased the capabilities for preclinical researches. However, most commercial and prototype optical imaging systems are camera-based systems using epi- or trans- illumination mode, with limited views of small animals. And for more accurate tomographic image reconstruction, additional data and information of a target animal is necessary. To overcome these issues, researchers have suggested several approaches such as maximizing the detection area or using the information of other highresolution modalities such as CT, MRI or Ultrasound, or using multi-spectral signals. As one of ways to maximizing the detection area of a target animal, we present a new fluorescence and bioluminescence imaging system for small animals, which can image entire surface of a target animal simultaneously. This system uses double mirror reflection scheme and it consists of input unit, imaging unit with two conical mirrors, the source illumination part and the surface scanner, and the detection unit with an intensified CCD camera system. Two conical mirrors are configured that a larger size mirror captures a target animal surface, and a smaller size mirror projects this captured image onto a CCD camera with one acquisition. With this scheme, we could capture entire surface of a target animal simultaneously and improve back reflection issue between a mirror and an animal surface of a single conical mirror scheme. Additionally, we could increase accessibility to an animal for multi-modality integration by providing unobstructed space around a target animal.

Paper Details

Date Published: 25 March 2013
PDF: 6 pages
Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 857821 (25 March 2013); doi: 10.1117/12.2005487
Show Author Affiliations
Jong Hwan Lee, Columbia Univ. (United States)
Hyun Keol Kim, Columbia Univ. Medical Ctr. (United States)
Jingfei Jia, Columbia Univ. (United States)
Christopher Fong, Columbia Univ. (United States)
Andreas H. Hielscher, Columbia Univ. (United States)
Columbia Univ. Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 8578:
Optical Tomography and Spectroscopy of Tissue X
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca, Editor(s)

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