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Proceedings Paper

Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo
Author(s): Xin Cai; Yu Zhang; Li Li; Sung-Wook Choi; Matthew R. MacEwan; Junjie Yao; Chulhong Kim; Younan Xia; Lihong V. Wang
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Paper Abstract

It is a challenge to non-invasively visualize in vivo the neovascularization in a three-dimensional (3D) scaffold with high spatial resolution and deep penetration depth. Here we used photoacoustic microscopy (PAM) to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model for up to six weeks. The neovasculature was observed to develop gradually in the same mouse. These blood vessels not only grew on top of the implanted scaffold but also penetrated into the scaffold. The PAM system offered a lateral resolution of ~45 μm and a penetration depth of ~3 mm into the scaffold/tissue construct. By using the 3D PAM data, we further quantified the vessel area as a function of time.

Paper Details

Date Published: 4 March 2013
PDF: 6 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858128 (4 March 2013); doi: 10.1117/12.2005236
Show Author Affiliations
Xin Cai, Washington Univ. in St. Louis (United States)
Yu Zhang, Georgia Institute of Technology and Emory Univ. (United States)
Li Li, Massachusetts General Hospital and Harvard Medical School (United States)
Sung-Wook Choi, The Catholic Univ. of Korea (Korea, Republic of)
Matthew R. MacEwan, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Chulhong Kim, Univ. at Buffalo, SUNY (United States)
Younan Xia, Georgia Institute of Technology and Emory Univ. (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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