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Proceedings Paper

High quality optical microangiography of ocular microcirculation and measurement of total retinal blood flow in mouse eye
Author(s): Zhongwei Zhi; Xin Yin; Suzan Dziennis; Charles E. Alpers; Ruikang K. Wang
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Paper Abstract

Visualization and measurement of retinal blood flow (RBF) is important to the diagnosis and management of different eye diseases, including diabetic retinopathy. Optical microangiography (OMAG) is developed for generating 3D dynamic microcirculation image and later refined into ultra-high sensitive OMAG (UHS-OMAG) for true capillary vessels imaging. Here, we present the application of OMAG imaging technique for visualization of depth-resolved vascular network within retina and choroid as well as measurement of total retinal blood flow in mice. A fast speed spectral domain OCT imaging system at 820nm with a line scan rate of 140 kHz was developed to image mouse posterior eye. By applying UHS-OMAG scanning protocol and processing algorithm, we achieved true capillary level imaging of retina and choroid vasculature in mouse eye. The vascular pattern within different retinal layers and choroid was presented. An en face Doppler OCT approach [1] without knowing Doppler angle was adopted for the measurement of total retinal blood flow. The axial blood flow velocity is measured in an en face plane by raster scanning and the flow is calculated by integrating over the vessel area of the central retinal artery.

Paper Details

Date Published: 26 March 2013
PDF: 6 pages
Proc. SPIE 8567, Ophthalmic Technologies XXIII, 856728 (26 March 2013); doi: 10.1117/12.2005222
Show Author Affiliations
Zhongwei Zhi, Univ. of Washington (United States)
Xin Yin, Univ. of Washington (United States)
Suzan Dziennis, Univ. of Washington (United States)
Charles E. Alpers, Univ. of Washington (United States)
Ruikang K. Wang, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 8567:
Ophthalmic Technologies XXIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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