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Proceedings Paper

Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia
Author(s): Rahul Pal; Kert Edward; Tyra Brown; Liang Ma; Jinping Yang; Susan McCammon; Massoud Motamedi; Gracie Vargas
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Paper Abstract

Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) have shown the potential for noninvasive assessment of oral precancers and cancers. We have explored a combination of these nonlinear optical microscopic imaging techniques with widefield fluorescence imaging to assess morphometry similar to that of pathologic evaluation as well as information from endogenous fluorophores, which are altered with neoplastic transformation. Widefield fluorescence revealed areas of interest corresponding to sites with precancers or early tumors, generally resulting in a decrease in green emission or increase in red emission. Subsequent microscopy revealed significant differences in morphology between normal, dysplastic/neoplastic mucosa for all layers. Combination of a widefield and a microscopic technique provides a novel approach for tissue morphometric analysis along with large area assessment of tissue autofluorescence properties.

Paper Details

Date Published: 22 March 2013
PDF: 6 pages
Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 85720M (22 March 2013); doi: 10.1117/12.2005144
Show Author Affiliations
Rahul Pal, The Univ. of Texas Medical Branch (United States)
Kert Edward, The Univ. of the West Indies (Jamaica)
Tyra Brown, The Univ. of Texas Medical Branch (United States)
Liang Ma, The Univ. of Texas Medical Branch (United States)
Jinping Yang, The Univ. of Texas Medical Branch (United States)
Susan McCammon, The Univ. of Texas Medical Branch (United States)
Massoud Motamedi, The Univ. of Texas Medical Branch (United States)
The Univ. of the West Indies (Jamaica)
Gracie Vargas, The Univ. of Texas Medical Branch (United States)


Published in SPIE Proceedings Vol. 8572:
Advanced Biomedical and Clinical Diagnostic Systems XI
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest, Editor(s)

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