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Proceedings Paper

Role of cytoskeleton and elastic moduli in cellular response to nanosecond pulsed electric fields
Author(s): Gary Lee Thompson; Caleb Roth; Gleb Tolstykh; Marjorie Kuipers; Bennett L. Ibey
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Paper Abstract

Nanosecond pulsed electric fields (nsPEFs) are known to increase cell membrane permeability to small molecules in accordance with dosages. As previous work has focused on nsPEF exposures in whole cells, electrodeformation may contribute to this induced-permeabilization in addition to other biological mechanisms. Here, we hypothesize that cellular elasticity, based upon the cytoskeleton, affects nsPEF-induced decrease in cellular viability. Young’s moduli of various types of cells have been calculated from atomic force microscopy (AFM) force curve data, showing that CHO cells are stiffer than non-adherent U937 and Jurkat cells, which are more susceptible to nsPEF exposure. To distinguish any cytoskeletal foundation for these observations, various cytoskeletal reagents were applied. Inhibiting actin polymerization significantly decreased membrane integrity, as determined by relative propidium uptake and phosphatidylserine externalization, upon exposure at 150 kV/cm with 100 pulses of 10 ns pulse width. Exposure in the presence of other drugs resulted in insignificant changes in membrane integrity and 24-hour viability. However, Jurkat cells showed greater lethality than latrunculin-treated CHO cells of comparable elasticity. From these results, it is postulated that cellular elasticity rooted in actin-membrane interaction is only a minor contributor to the differing responses of adherent and non-adherent cells to nsPEF insults.

Paper Details

Date Published: 23 February 2013
PDF: 9 pages
Proc. SPIE 8585, Terahertz and Ultrashort Electromagnetic Pulses for Biomedical Applications, 85850T (23 February 2013); doi: 10.1117/12.2005114
Show Author Affiliations
Gary Lee Thompson, National Research Council (United States)
Caleb Roth, Univ. of Texas Health Science Ctr. at San Antonio (United States)
Gleb Tolstykh, National Research Council (United States)
Marjorie Kuipers, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 8585:
Terahertz and Ultrashort Electromagnetic Pulses for Biomedical Applications
Gerald J. Wilmink; Bennett L. Ibey, Editor(s)

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