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Proceedings Paper

Optimized release matrices for use in a BioMEMS device to study metastasis
Author(s): Ashley Clark; James Williams; Michael Padgen; Patricia Keely; John Condeelis; James Castracane
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Paper Abstract

Multiple changes within the tumor microenvironment have been correlated with an increase in metastasis, yet the mechanisms are not fully understood. Tumor cells can be stimulated by the release of chemoattractant factors such as epidermal growth factor (EGF) from nearby stromal cells, resulting in increased intravasation and metastasis. Additionally, altered extracellular matrix density can result in changes in gene expression patterns governing increased cellular proliferation and motility. The Nano Intravital Device (NANIVID) has been used to produce gradients of select soluble factors in the tumor microenvironment and to study the role of these changes on cell migration. In previous studies, the NANIVID utilized a synthetic hydrogel to produce an EGF gradient to attract metastatic breast cancer cells. In this work, a matrigel insert will be introduced into the outlet to provide a substrate for cells to migrate on when entering the device. The concentration of the chemoattractant and matrigel comprising the insert will be optimized to produce a suitable gradient for inducing chemotaxis in metastatic breast cancer cells in vitro. Additionally, silk and alginate matrices will be explored as improved soluble factor release mediums. Delivery of larger molecules such as collagen cross-linkers requires an alternative hydrogel material. Future NANIVID experiments will utilize these materials to gauge the cellular motility response when a stiffer matrix is encountered.

Paper Details

Date Published: 13 March 2013
PDF: 7 pages
Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150C (13 March 2013); doi: 10.1117/12.2005048
Show Author Affiliations
Ashley Clark, Univ. at Albany (United States)
James Williams, Univ. at Albany (United States)
Michael Padgen, Univ. at Albany (United States)
Patricia Keely, Univ. of Wisconsin-Madison (United States)
John Condeelis, Yeshiva Univ. (United States)
James Castracane, Univ. at Albany (United States)


Published in SPIE Proceedings Vol. 8615:
Microfluidics, BioMEMS, and Medical Microsystems XI
Holger Becker; Bonnie L. Gray, Editor(s)

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