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Proceedings Paper

A dual-reporter fluorescent imaging approach can be used to estimate sentinel lymph node tumor burden
Author(s): Kenneth M. Tichauer; Kimberley S. Samkoe; Jason R. Gunn; Ramesh Govindan; Aravind Viswanathan; P. Jack Hoopes; Tayyaba Hasan; Peter A. Kaufman; Brian W. Pogue
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Paper Abstract

The presence of metastatic tumor cells in tumor-draining lymph nodes is an important indicator for cancer staging and therapy. Current clinical approaches of assessing lymph node tumor burden require invasive surgery that can be associated with nerve damage and other complications. In this study, a dual-reporter fluorescence molecular imaging approach, previously validated for quantifying targeted reporter binding in various human tumor xenographs, was assessed as a means of quantifying tumor burden in metastatic disease in mice. The utility of the dual-reporter imaging approach to measure tumor burden in sentinel lymph nodes was investigated in a bioluminescent human breast cancer xenograph model in 18 female nude mice. Once the presence of tumor in the lymph node was confirmed by bioluminescent imaging, fluorescently labeled anti-EGFR antibody and an untargeted antibody (labeled with a different fluorophore) were injected intradermally, proximal to the lymph node, and the uptake of the two reporters was imaged simultaneously with a with a flat-panel fluorescent scanner. Preliminary results demonstrated a statistically significant correlation between the dual-reporter measured tumor burden and the bioluminescent measure of tumor burden.

Paper Details

Date Published: 25 March 2013
PDF: 7 pages
Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 85781V (25 March 2013); doi: 10.1117/12.2004913
Show Author Affiliations
Kenneth M. Tichauer, Illinois Institute of Technology (United States)
Thayer School of Engineering at Dartmouth (United States)
Kimberley S. Samkoe, Thayer School of Engineering at Dartmouth (United States)
Geisel School of Medicine (United States)
Jason R. Gunn, Thayer School of Engineering at Dartmouth (United States)
Ramesh Govindan, Thayer School of Engineering at Dartmouth (United States)
Aravind Viswanathan, Thayer School of Engineering at Dartmouth (United States)
P. Jack Hoopes, Thayer School of Engineering at Dartmouth (United States)
Geisel School of Medicine (United States)
Tayyaba Hasan, The Wellman Institute of Photomedicine, Massachusetts General Hospital (United States)
Peter A. Kaufman, Geisel School of Medicine (United States)
Brian W. Pogue, Thayer School of Engineering at Dartmouth (United States)
Geisel School of Medicine (United States)
The Wellman Institute of Photomedicine, Massachusetts General Hospital (United States)


Published in SPIE Proceedings Vol. 8578:
Optical Tomography and Spectroscopy of Tissue X
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca, Editor(s)

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