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Proceedings Paper

Quantitative longitudinal measurement in a rat model of controlled burn severity using spatial frequency domain imaging
Author(s): John Quan Nguyen; Christian Crouzet; Tuan Mai; Kathleen Riola; Daniel Uchitel; Lih-Huei Liaw; Nicole Bernal; Adrien Ponticorvo; Bernard Choi; Anthony J. Durkin
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Paper Abstract

Background and Objective: Spatial Frequency Domain Imaging (SFDI) is a non-contact wide-field optical imaging technology currently being developed to investigate the feasibility of quantitative non-invasive evaluation of burn wound severity in a rat model. Our objective is to determine the potential of SFDI for mapping quantitative changes in spatially resolved tissue oxygen saturation and water concentration may be indicative of burn wound severity, healing, and further complications. In this portion of the investigation, we focus on the development of a rat burn model and the acute response of tissue to burn wounds. Study Design/Materials and Methods: A controlled burn protocol involving a heated brass comb was applied to 6 rats. Imaging was acquired at 17 evenly spaced wavelengths in the near-infrared from 650 to 970 nm. Over the course of the 3 hour post-burn period, we were able to map quantitative changes in spatially resolved chromophores. Burn severities were verified post-experiment using standard H and E histology and optical microscopy. Results/Conclusion: In total, we were able to induce 12 superficial-partial thickness burns, 8 deep-partial thickness burns, and 4 full thickness burns in our rat models. While several tissue chromophores were tracked, we found that changes in oxygen saturation and water concentration to be sensitive indicators of burn severity. Future work will include additional longitudinal studies over a period of days in order to investigate which parameters are correlated to tissue healing.

Paper Details

Date Published: 8 March 2013
PDF: 7 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85650S (8 March 2013); doi: 10.1117/12.2004894
Show Author Affiliations
John Quan Nguyen, Beckman Laser Institute and Medical Clinic (United States)
Christian Crouzet, Beckman Laser Institute and Medical Clinic (United States)
Tuan Mai, Beckman Laser Institute and Medical Clinic (United States)
Kathleen Riola, Beckman Laser Institute and Medical Clinic (United States)
Daniel Uchitel, Beckman Laser Institute and Medical Clinic (United States)
Lih-Huei Liaw, Beckman Laser Institute and Medical Clinic (United States)
Nicole Bernal, Univ. of California, Irvine Medical Ctr. (United States)
Adrien Ponticorvo, Beckman Laser Institute and Medical Clinic (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Anthony J. Durkin, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory; Guillermo J. Tearney; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen; E. Duco Jansen; Justus F. Ilgner; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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