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Proceedings Paper

Ultra-high frequency ultrasound biomicroscopy and high throughput cardiovascular phenotyping in a large scale mouse mutagenesis screen
Author(s): Xiaoqin Liu; Richard Francis; Kimimasa Tobita; Andy Kim; Linda Leatherbury; Cecilia W. Lo
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Paper Abstract

Ultrasound biomicroscopy (UBM) is ideally suited for phenotyping fetal mice for congenital heart disease (CHD), as imaging can be carried out noninvasively to provide both hemodynamic and structural information essential for CHD diagnosis. Using the UBM (Vevo 2100; 40Hz) in conjunction with the clinical ultrasound system (Acuson Sequioa C512; 15Hz), we developed a two-step screening protocol to scan thousands fetuses derived from ENU mutagenized pedigrees. A wide spectrum of CHD was detected by the UBM, which were subsequently confirmed with follow-up necropsy and histopathology examination with episcopic fluorescence image capture. CHD observed included outflow anomalies, left/right heart obstructive lesions, septal/valvular defects and cardiac situs anomalies. Meanwhile, various extracardiac defects were found, such as polydactyly, craniofacial defects, exencephaly, omphalocele,cleft palate, most of which were associated with cardiac defects. Our analyses showed the UBM was better at assessing cardiac structure and blood flow profiles, while conventional ultrasound allowed higher throughput low-resolution screening. Our study showed the integration of conventional clinical ultrasound imaging with the UBM for fetal mouse cardiovascular phenotyping can maximize the detection and recovery of CHD mutants.

Paper Details

Date Published: 26 March 2013
PDF: 8 pages
Proc. SPIE 8593, Optical Methods in Developmental Biology, 859309 (26 March 2013); doi: 10.1117/12.2004860
Show Author Affiliations
Xiaoqin Liu, Univ. of Pittsburgh School of Medicine (United States)
Richard Francis, Univ. of Pittsburgh School of Medicine (United States)
Kimimasa Tobita, Univ. of Pittsburgh School of Medicine (United States)
Andy Kim, Univ. of Pittsburgh School of Medicine (United States)
Linda Leatherbury, Children's National Medical Ctr. (United States)
Cecilia W. Lo, Univ. of Pittsburgh School of Medicine (United States)

Published in SPIE Proceedings Vol. 8593:
Optical Methods in Developmental Biology
Andrew M. Rollins; Cecilia Lo; Scott E. Fraser, Editor(s)

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