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Proceedings Paper

Multimodal optoacoustic and multiphoton fluorescence microscopy
Author(s): Gali Sela; Daniel Razansky; Shy Shoham
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Paper Abstract

Multiphoton microscopy is a powerful imaging modality that enables structural and functional imaging with cellular and sub-cellular resolution, deep within biological tissues. Yet, its main contrast mechanism relies on extrinsically administered fluorescent indicators. Here we developed a system for simultaneous multimodal optoacoustic and multiphoton fluorescence 3D imaging, which attains both absorption and fluorescence-based contrast by integrating an ultrasonic transducer into a two-photon laser scanning microscope. The system is readily shown to enable acquisition of multimodal microscopic images of fluorescently labeled targets and cell cultures as well as intrinsic absorption-based images of pigmented biological tissue. During initial experiments, it was further observed that that detected optoacoustically-induced response contains low frequency signal variations, presumably due to cavitation-mediated signal generation by the high repetition rate (80MHz) near IR femtosecond laser. The multimodal system may provide complementary structural and functional information to the fluorescently labeled tissue, by superimposing optoacoustic images of intrinsic tissue chromophores, such as melanin deposits, pigmentation, and hemoglobin or other extrinsic particle or dye-based markers highly absorptive in the NIR spectrum.

Paper Details

Date Published: 4 March 2013
PDF: 4 pages
Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85812H (4 March 2013); doi: 10.1117/12.2004815
Show Author Affiliations
Gali Sela, Technion-Israel Institute of Technology (Israel)
Daniel Razansky, Technical Univ. of Munich and Helmholtz Ctr. Munich (Germany)
Shy Shoham, Technion-Israel Institute of Technology (Israel)


Published in SPIE Proceedings Vol. 8581:
Photons Plus Ultrasound: Imaging and Sensing 2013
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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