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Proceedings Paper

Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers
Author(s): Christian Witte; Clemens Kremer; Jonathan M. Cooper; Steven L. Neale
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Paper Abstract

A versatile platform for efficient cell lysis using a combination of acoustic and electric fields in a microchannel is presented. Cell membrane disruption is triggered by electric fields inducing electroporation and then lysis. The principle of optoelectronic tweezers (OET) is applied to control the electric field strength and a surface acoustic wave transducer is attached to an OET chip to implement acoustic tweezing (AT). The system is characterized in terms of spatial control of electric fields, single cell precision and lysis times. Under continuous operation, a combination of AT and OET improves cell lysis significantly achieving for sample concentrations of 106 cell/ml lysis efficiencies of > 99%.

Paper Details

Date Published: 9 March 2013
PDF: 8 pages
Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150T (9 March 2013); doi: 10.1117/12.2004814
Show Author Affiliations
Christian Witte, The Univ. of Glasgow (United Kingdom)
Clemens Kremer, The Univ. of Glasgow (United Kingdom)
Jonathan M. Cooper, The Univ. of Glasgow (United Kingdom)
Steven L. Neale, The Univ. of Glasgow (United Kingdom)

Published in SPIE Proceedings Vol. 8615:
Microfluidics, BioMEMS, and Medical Microsystems XI
Holger Becker; Bonnie L. Gray, Editor(s)

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