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Proceedings Paper

Assessing tumor physiology by dynamic contrast-enhanced near-infrared spectroscopy
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Paper Abstract

The purpose of this study was to develop a dynamic contrast-enhanced (DCE) near-infrared spectroscopy (NIRS) technique to characterize tumor physiology. Dynamic data were acquired using two contrast agents of different molecular weights, indocyanine green (ICG) and IRDye 800CW carboxylate (IRDcxb). The DCE curves were analyzed using a kinetic model capable of extracting estimates of tumor blood flow (F), capillary transit time (tc) and the amount of dye that leaked into the extravascular space (EVS) – characterized by the extraction fraction (E). Data were acquired from five nude rats with tumor xenografts (>10mm) implanted in the neck. Four DCE-NIR datasets (two from each contrast agent) were acquired for each rat. The dye concentration curve in arterial blood, which is required to quantify the model parameters, was measured non-invasively by dye densitometry. A modification to the kinetic model to characterize tc as a distribution of possible values, rather than finite, improved the fit of acquired tumor concentration curves, resulting in more reliable estimates. This modified kinetic model identified a difference between the extracted fraction of IRDcxb, 15 ± 6 %, and ICG, 1.6 ± 0.6 %, in the tumor, which can be explained by the difference in molecular weight: 67 kDa for ICG since it binds to albumin and 1.17 kDa for IRD. This study demonstrates the ability of DCENIRS to quantify tumor physiology. The next step is to adapt this approach with a dual-receptor approach.

Paper Details

Date Published: 25 March 2013
PDF: 9 pages
Proc. SPIE 8578, Optical Tomography and Spectroscopy of Tissue X, 857823 (25 March 2013); doi: 10.1117/12.2004774
Show Author Affiliations
Kyle Verdecchia, Lawson Health Research Institute (Canada)
Western Univ. (Canada)
Jonathan Elliott, Lawson Health Research Institute (Canada)
Western Univ. (Canada)
Mamadou Diop, Lawson Health Research Institute (Canada)
Western Univ. (Canada)
Lisa Hoffman, Lawson Health Research Institute (Canada)
Western Univ. (Canada)
Ting-Yim Lee, Lawson Health Research Institute (Canada)
Western Univ. (Canada)
Robarts Research Institute (Canada)
Keith St. Lawrence, Lawson Health Research Institute (Canada)
Western Univ. (Canada)


Published in SPIE Proceedings Vol. 8578:
Optical Tomography and Spectroscopy of Tissue X
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca, Editor(s)

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