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Proceedings Paper

Effects of oxygenation on PDT efficacy as a function of reactive oxygen species formation
Author(s): David Kessel; Michael Price
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Paper Abstract

Using two agents that preferentially target lysosomes for photodamage but produce different reactive oxygen species (ROS) upon irradiation, we examined effects of the degree of oxygenation on photokilling. Irradiation of the chlorin NPe6 results in a high yield of singlet oxygen but the bacteriopheophorbide WST11 forms only oxygen radicals. We found that PDT efficacy of WST11 was impaired when the ambient oxygen concentration was reduced to 1%, while that of NPe6 was essentially unaffected. These result were correlated with photobleaching effects. Use of fluorescent probes for singlet oxygen vs. OH radical revealed that photobleaching was correlated with 1O2 but not .OH formation during irradiation of solutions containing NPe6. Photoproduct formation could be followed by changes in absorbance spectra. In the presence of mercaptoethanol, different photoproducts were formed, showing that environmental factors could influence photochemical reactions.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, 856802 (22 February 2013); doi: 10.1117/12.2004560
Show Author Affiliations
David Kessel, Wayne State Univ. School of Medicine (United States)
Michael Price, Wayne State Univ. School of Medicine (United States)


Published in SPIE Proceedings Vol. 8568:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII
David H. Kessel; Tayyaba Hasan, Editor(s)

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