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Proceedings Paper

Fluorescence lifetime imaging microscopy of nanodiamonds in vivo
Author(s): Yung Kuo; Tsung-Yuan Hsu; Yi-Chun Wu; Jui-Hung Hsu; Huan-Cheng Chang
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Paper Abstract

The negatively charged nitrogen-vacancy (NV) center in bulk diamond is a photostable fluorophore with a radiative lifetime of 11.6 ns at room temperature. The lifetime substantially increases to ~20 ns for diamond nanoparticles (size ~ 100 nm) suspended in water due to the change in refractive index of the surrounding medium of the NV centers. This fluorescence decay time is much longer than that (typically 1 − 4 ns) of endogenous and exogenous fluorophores commonly used in biological imaging, making it possible to detect NV-containing nanodiamonds in vivo at the single particle level by fluorescence lifetime imaging microscopy (FLIM). We demonstrate the feasibility of this approach using Caenorhabditis elegans (C. elegans) as a model organism.

Paper Details

Date Published: 29 March 2013
PDF: 7 pages
Proc. SPIE 8635, Advances in Photonics of Quantum Computing, Memory, and Communication VI, 863503 (29 March 2013); doi: 10.1117/12.2004494
Show Author Affiliations
Yung Kuo, Institute of Atomic and Molecular Sciences (Taiwan)
Tsung-Yuan Hsu, National Taiwan Univ. (Taiwan)
Yi-Chun Wu, National Taiwan Univ. (Taiwan)
Jui-Hung Hsu, National Sun Yat-Sen Univ. (Taiwan)
Huan-Cheng Chang, Institute of Atomic and Molecular Sciences (Taiwan)


Published in SPIE Proceedings Vol. 8635:
Advances in Photonics of Quantum Computing, Memory, and Communication VI
Zameer U. Hasan; Philip R. Hemmer; Hwang Lee; Charles M. Santori, Editor(s)

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