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Proceedings Paper

Study of virus by Raman spectroscopy
Author(s): K. Moor; H. Kitamura; K. Hashimoto; M. Sawa; B. B. Andriana; K. Ohtani ; T. Yagura; H. Sato
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Paper Abstract

Problem of viruses is very actual for nowadays. Some viruses, which are responsible for human of all tumors, are about 15 %. Main purposes this study, early detection virus in live cell without labeling and in the real time by Raman spectroscopy. Micro Raman spectroscopy (mRs) is a technique that uses a Raman spectrometer to measure the spectra of microscopic samples. According to the Raman spectroscopy, it becomes possible to study the metabolites of a live cultured cell without labeling. We used mRs to detect the virus via HEK 293 cell line-infected adenovirus. We obtained raman specters of lives cells with viruses in 24 hours and 7 days after the infection. As the result, there is some biochemical changing after the treatment of cell with virus. One of biochemical alteration is at 1081 cm-1. For the clarification result, we use confocal fluorescent microscopy and transmission electron microscopy (TEM).

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871X (22 February 2013); doi: 10.1117/12.2004476
Show Author Affiliations
K. Moor, Kwansei Gakuin Univ. (Japan)
H. Kitamura, Kwansei Gakuin Univ. (Japan)
K. Hashimoto, Kwansei Gakuin Univ. (Japan)
M. Sawa, Kwansei Gakuin Univ. (Japan)
B. B. Andriana, Kwansei Gakuin Univ. (Japan)
K. Ohtani , Kwansei Gakuin Univ. (Japan)
T. Yagura, Kwansei Gakuin Univ. (Japan)
H. Sato, Kwansei Gakuin Univ. (Japan)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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