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Proceedings Paper

Supercritical scattering microscoy for quantitative phase in the vicinity of a lamella
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Paper Abstract

In this paper, we discuss the possibility of making a super-axially-resolved image of a biological sample using supercritical angle diffusion. This labeling-free approach is suitable to any microscope equipped with a NAobj < 1.33 microscope objective and can be used either for conventional intensity imaging or for quantitative phase imaging. We expose some results on beads an cells showing the potential of this method.

Paper Details

Date Published: 22 February 2013
PDF: 5 pages
Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858916 (22 February 2013); doi: 10.1117/12.2004130
Show Author Affiliations
Pierre Bon, Institut Langevin, ESPCI, CNRS (France)
Institut des Sciences Moléculaires d'Orsay and Centre de photonique Biomédicale, CNRS, Paris-11 Univ (France)
Thomas Barroca, Institut Langevin, ESPCI, CNRS (France)
Sandrine Lévêque-Fort, Institut des Sciences Moléculaires d'Orsay, CNRS, Univ. Paris-Sud (France)
Emmanuel Fort, Institut Langevin, ESPCI, CNRS (France)

Published in SPIE Proceedings Vol. 8589:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson, Editor(s)

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