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Proceedings Paper

Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT
Author(s): WooJhon Choi; Bernhard Baumann; Allen C. Clermont; Edward P. Feener; David A. Boas; James G. Fujimoto
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Paper Abstract

Measuring retinal hemodynamics in response to flicker stimulus is important for investigating pathophysiology in small animal models of diabetic retinopathy, because a reduction in the hyperemic response is thought to be one of the earliest changes in diabetic retinopathy. In this study, we investigated functional imaging of retinal hemodynamics in response to flicker stimulus in the rat retina using an ultrahigh speed spectral / Fourier domain OCT system at 840nm with an axial scan rate of 244kHz. At 244kHz the nominal axial velocity range that could be measured without phase wrapping was +/-37.7mm/s. Pulsatile total retinal arterial blood flow as a function of time was measured using an en face Doppler approach where a 200μm × 200μm area centered at the central retinal artery was repeatedly raster scanned at a volume acquisition rate of 55Hz. Three-dimensional capillary imaging was performed using speckle decorrelation which has minimal angle dependency compared to other angiography techniques based on OCT phase information. During OCT imaging, a flicker stimulus could be applied to the retina synchronously by inserting a dichroic mirror in the imaging interface. An acute transient increase in total retinal blood flow could be detected. At the capillary level, an increase in the degree of speckle decorrelation in capillary OCT angiography images could also be observed, which indicates an increase in the velocity of blood at the capillary level. This method promises to be useful for the investigation of small animal models of ocular diseases.

Paper Details

Date Published: 26 March 2013
PDF: 6 pages
Proc. SPIE 8567, Ophthalmic Technologies XXIII, 85671C (26 March 2013); doi: 10.1117/12.2004096
Show Author Affiliations
WooJhon Choi, Massachusetts Institute of Technology (United States)
Bernhard Baumann, Massachusetts Institute of Technology (United States)
Tufts Univ. (United States)
Allen C. Clermont, Harvard Medical School (United States)
Edward P. Feener, Harvard Medical School (United States)
David A. Boas, Massachusetts General Hospital (United States)
James G. Fujimoto, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 8567:
Ophthalmic Technologies XXIII
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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