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Proceedings Paper

Microstructured optical fiber Bragg grating sensor for DNA detection
Author(s): A. Candiani; S. Giannetti; M. Sozzi; E. Coscelli; F. Poli; A. Cucinotta; A. Bertucci; R. Corradini; M. Konstantaki; W. Margulis; S. Pissadakis; S. Selleri
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Paper Abstract

In this work the inner surface of a microstructured optical fiber, where a Bragg grating was previously inscribed, has been functionalized using peptide nucleic acid probe targeting a DNA sequence of the cystic fibrosis disease. The solution of DNA molecules, matched with the PNA probes, has been infiltrated inside the fiber capillaries and hybridization has been realized according to the Watson - Crick Model. In order to achieve signal amplification, oligonucleotide-functionalized gold nanoparticles were then infiltrated and used to form a sandwich-like system. Experimental measurements show a clear wavelength shift of the reflected high order mode for a 100 nM DNA solution. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation and proving a good reproducibility of the results, suggesting the possibility of the reuse of the sensor. Measurements have been also made using a 100 nM mis-matched DNA solution, containing a single nucleotide polymorphism, demonstrating the high selectivity of the sensor.

Paper Details

Date Published: 20 March 2013
PDF: 9 pages
Proc. SPIE 8576, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII, 85760E (20 March 2013); doi: 10.1117/12.2003997
Show Author Affiliations
A. Candiani, Univ. degli Studi di Parma (Italy)
S. Giannetti, Univ. degli Studi di Parma (Italy)
M. Sozzi, Univ. degli Studi di Parma (Italy)
E. Coscelli, Univ. degli Studi di Parma (Italy)
F. Poli, Univ. degli Studi di Parma (Italy)
A. Cucinotta, Univ. degli Studi di Parma (Italy)
A. Bertucci, Univ. degli Studi di Parma (Italy)
R. Corradini, Univ. degli Studi di Parma (Italy)
M. Konstantaki, Foundation for Research and Technology - Hellas (Greece)
W. Margulis, Acreo AB (Sweden)
S. Pissadakis, Foundation for Research and Technology - Hellas (Greece)
S. Selleri, Univ. degli Studi di Parma (Italy)


Published in SPIE Proceedings Vol. 8576:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIII
Israel Gannot, Editor(s)

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