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Proceedings Paper

Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells
Author(s): M. L. Torres-Mapa; J. Gardner; H. Bradburn; J. King; K. Dholakia; F. Gunn-Moore
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Paper Abstract

We demonstrate the use of femtosecond optical transfection for the genetic manipulation of human embryonic stem cells. Using a system with an SLM combined with a scanning mirror allows poration of both single-cell and colony-formed human embryonic stem cells in a rapid and targeted manner. In this work, we show successful transfection of plasmid DNA tagged with fluorescent reporters into human embryonic stem cells using three doses of focused femtosecond laser. A significant number of transfected cells retained their undifferentiated morphological feature of large nucleus with high nucleus to cytoplasmic ratio, 48h after photoporation. Furthermore, DNA constructs driven by different types of promoters were also successfully transfected into human embryonic stem cells using this technique.

Paper Details

Date Published: 15 March 2013
PDF: 7 pages
Proc. SPIE 8611, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII, 861104 (15 March 2013); doi: 10.1117/12.2003739
Show Author Affiliations
M. L. Torres-Mapa, Univ. of St. Andrews (United Kingdom)
J. Gardner, Roslin Cellab (United Kingdom)
H. Bradburn, Roslin Cellab (United Kingdom)
J. King, Roslin Cellab (United Kingdom)
K. Dholakia, Univ. of St. Andrews (United Kingdom)
F. Gunn-Moore, Univ. of St. Andrews (United Kingdom)


Published in SPIE Proceedings Vol. 8611:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII
Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte, Editor(s)

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