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Proceedings Paper

Supercritical self-interference fluorescence microscopy for full-field membrane imaging
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Paper Abstract

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (22 February 2013); doi: 10.1117/12.2003736
Show Author Affiliations
Thomas Barroca, Institut Langevin, CNRS, ESPCI ParisTech (France)
Pierre Bon, Institut Langevin, CNRS, ESPCI ParisTech (France)
Institut des Sciences Moléculaires d'Orsay and Ctr. de photonique Biomédicale, CNRS, Univ. Paris-Sud (France)
Sandrine Lévêque-Fort, Institut des Sciences Moléculaires d'Orsay and Ctr. de photonique Biomédicale, CNRS, Univ. Paris-Sud (France)
Emmanuel Fort, Institut Langevin, CNRS, ESPCI ParisTech (France)


Published in SPIE Proceedings Vol. 8589:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson, Editor(s)

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