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Proceedings Paper

ICG-loaded polymeric nanocapsules functionalized with anti-HER2 for targeted fluorescence imaging and photodestruction of ovarian cancer cells
Author(s): Baharak Bahmani; Yadir Guerrero; Valentine Vullev; Sheela P. Singh; Vikas Kundra; Bahman Anvari
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Paper Abstract

Optical nano-materials present a promising platform for targeted molecular imaging of cancer biomarkers and its photodestruction. Our group is investigating the use of polymeric nanoparticles, loaded with indocyanine green, an FDA-approved chromophore, as a theranostic agent for targeted intraoperative optical imaging and laser-mediated destruction of ovarian cancer. These ICG-loaded nanocapsules (ICG-NCs) can be functionalized by covalent attachment of targeting moieties onto their surface. Here, we investigate ICG-NCs functionalized with anti-HER2 for targeted fluorescence imaging and laser-mediated destruction of ovarian cancer cells in vitro. ICG-NCs are formed through ionic cross-linking between polyallylamine hydrochloride chains and sodium phosphate ions followed by diffusion-mediated loading with ICG. Before functionalization with antibodies, the surface of ICG-NCs is coated with single and double aldehyde terminated polyethylene glycol (PEG). The monoclonal anti-HER2 is covalently coupled to the PEGylated ICG-NCs using reductive amination to target the HER2 receptor, a biomarker whose over-expression is associated with increased risk of cancer progression. We quantify uptake of anti-HER2 conjugated ICG-NCs by ovarian cancer cells using flow cytometery. The in-vitro laser-mediated destruction of SKOV3 cells incubated with anti-HER2 functionalized ICG-NCs is performed using an 808 nm diode laser. Cell viability is characterized using the Calcein and Ethidium homodimer-1 assays following laser irradiation. Our results indicate that anti-HER2 functionalized ICG-NCs can be used as theranostic agents for optical molecular imaging and photodestruction of ovarian cancers in-vitro.

Paper Details

Date Published: 15 March 2013
PDF: 5 pages
Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 859602 (15 March 2013); doi: 10.1117/12.2003453
Show Author Affiliations
Baharak Bahmani, Univ. of California, Riverside (United States)
Yadir Guerrero, Univ. of California, Riverside (United States)
Valentine Vullev, Univ. of California, Riverside (United States)
Sheela P. Singh, Univ. of Texas MD Anderson Cancer Ctr. (United States)
Vikas Kundra, Univ. of Texas MD Anderson Cancer Ctr. (United States)
Bahman Anvari, Univ. of California, Riverside (United States)


Published in SPIE Proceedings Vol. 8596:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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