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Proceedings Paper

Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope
Author(s): Pierre Bon; Shérazade Aknoun; Julien Savatier; Benoit Wattellier; Serge Monneret
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Paper Abstract

In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.

Paper Details

Date Published: 22 February 2013
PDF: 5 pages
Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858918 (22 February 2013); doi: 10.1117/12.2003426
Show Author Affiliations
Pierre Bon, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Institut Langevin, CNRS, Paris Diderot Univ. (France)
Shérazade Aknoun, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Phasics S.A. (France)
Julien Savatier, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Benoit Wattellier, Phasics S.A. (France)
Serge Monneret, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)


Published in SPIE Proceedings Vol. 8589:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX
Carol J. Cogswell; Thomas G. Brown; Jose-Angel Conchello; Tony Wilson, Editor(s)

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