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Proceedings Paper

Expression and function of channelrhodopsin 2 in mouse outer hair cells
Author(s): Fangyi Chen; Tao Wu; Teresa Wilson; Hrebesh Subhash; Irina Omelchenko; Michael Bateschell; Lingyan Wang; John Brigande; Zhi-Gen Jiang; Alfred Nuttall
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Paper Abstract

Outer hair cell (OHC) is widely accepted as the origin of cochlear amplification, a mechanism that accounts for the extreme sensitivity of the mammalian hearing. The key process of cochlear amplification is the reverse transduction, where the OHC changes its length under electrical stimulation. In this study, we developed a method to modulate electro-mechanical transduction with an optogenetic approach based on channelrhodopsin 2 (ChR2), a direct lightactivated non-selective cation channel (NSCC). We specifically expressed ChR2 in mouse cochlea OHCs through in uterus injection of adenovirus vector with ChR2 in fusion with the fluorescent marker tdTomato. We also transfected ChR2(H134R), a point mutant of ChR2, with plasmid to an auditory cell line (HEI-OC1). With whole cell recording, we found that blue light (470 nm) elicited a current with a reversal potential around zero in both mouse OHCs and HEI-OC1 cells and generated depolarization in both cell types.

Paper Details

Date Published: 8 March 2013
PDF: 4 pages
Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860I (8 March 2013); doi: 10.1117/12.2003114
Show Author Affiliations
Fangyi Chen, South Univ. of Science & Technology of China (United States)
Oregon Health and Science Univ. (United States)
Tao Wu, Oregon Health & Science Univ. (United States)
Teresa Wilson, Oregon Health & Science Univ. (United States)
Hrebesh Subhash, Oregon Health & Science Univ. (United States)
Irina Omelchenko, Oregon Health & Science Univ. (United States)
Michael Bateschell, Oregon Health & Science Univ. (United States)
Lingyan Wang, Oregon Health & Science Univ. (United States)
John Brigande, Oregon Health & Science Univ. (United States)
Zhi-Gen Jiang, Oregon Health & Science Univ. (United States)
Alfred Nuttall, Oregon Health & Science Univ. (United States)
Univ. of Michigan, Ann Arbor (United States)


Published in SPIE Proceedings Vol. 8586:
Optogenetics: Optical Methods for Cellular Control
Samarendra K. Mohanty; Nitish V. Thakor, Editor(s)

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