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Proceedings Paper

Multiphoton imaging to distinguish grana and starch inside an intact leaf
Author(s): Mei-Yu Chen; Guan-Yu Zhuo; Po-Fu Chen; Pei-Chun Wu; Tzu-Ming Liu; Shi-Wei Chu
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Paper Abstract

We have demonstrated a straightforward and noninvasive method to identify the distribution of grana and starch within an intact leaf. Grana and starch are the major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two photon fluorescence (2PF) and second harmonic generation (SHG) signals. Consequently, SHG is found on both grana and starch while 2PF from chlorophyll indicates the identity of grana.

Paper Details

Date Published: 22 February 2013
PDF: 6 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858822 (22 February 2013); doi: 10.1117/12.2003075
Show Author Affiliations
Mei-Yu Chen, National Taiwan Univ. (Taiwan)
Guan-Yu Zhuo, National Taiwan Univ. (Taiwan)
Po-Fu Chen, National Taiwan Univ. (Taiwan)
Pei-Chun Wu, National Taiwan Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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