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Proceedings Paper

Integrated fingerprint and high wavenumber confocal Raman spectroscopy for in vivo diagnosis of cervical precancer
Author(s): Shiyamala Duraipandian; Wei Zheng; Joseph Ng; Jeffrey J. H. Low; A. Ilancheran; Zhiwei Huang
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Paper Abstract

Raman spectroscopy is a vibrational spectroscopic technique capable of optically probing the compositional, conformational, and structural changes in the tissue associated with disease progression. The main goal of this work is to develop an integrated fingerprint (FP) and high wavenumber (HW) in vivo confocal Raman spectroscopy for simultaneous FP/HW tissue Raman spectral measurements. This work further explores the potential of integrated FP/HW Raman spectroscopy developed as a diagnostic tool for in vivo detection of cervical precancer. A total of 473 in vivo integrated FP/HW Raman spectra (340 normal and 133 precancer) were acquired from 35 patients within 1 s during clinical colposcopy. The major tissue Raman peaks are noticed around 854, 937, 1001, 1095, 1253, 1313, 1445, 1654, 2946 and 3400 cm-1, related to the molecular changes (e.g., proteins, lipids, glycogen, nucleic acids, water, etc.) that accompany the dysplastic transformation of tissue. The FP (800 - 1800 cm-1), HW (2800 - 3800 cm-1) and the integrated FP/HW Raman spectra were analyzed using partial least squares-discriminant analysis (PLS-DA) together with the leave-one patient-out, cross-validation. The developed PLS-DA classification models and receiver operating characteristics (ROC) curves for the FP, HW and integrated FP/HW spectroscopy further discloses that the performance of integrated FP/HW Raman spectroscopy is superior to that of all others in discriminating the dysplastic cervix. The results of this work indicate that the co-contributions of underlying rich biochemical information revealed by the complementary spectral modalities (FP and HW Raman) can improve the in vivo early diagnosis of cervical precancer at clinical colposcopy

Paper Details

Date Published: 22 March 2013
PDF: 7 pages
Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 85720Z (22 March 2013); doi: 10.1117/12.2003058
Show Author Affiliations
Shiyamala Duraipandian, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Joseph Ng, National Univ. Hospital and National Univ. of Singapore (Singapore)
Jeffrey J. H. Low, National Univ. Hospital and National Univ. of Singapore (Singapore)
A. Ilancheran, National Univ. Hospital and National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 8572:
Advanced Biomedical and Clinical Diagnostic Systems XI
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest, Editor(s)

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