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Proceedings Paper

In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy
Author(s): Takeshi Yasui; Ryosuke Tanaka; Eiji Hase; Shu-ichiro Fukushima; Tsutomu Araki
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Paper Abstract

Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in this study, we demonstrated in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy. We confirmed that changes in SHG vanishing patterns in the SHG images depended on the burn degree. The results imply that SHG microscopy can be used as a low-invasiveness, highly quantitative tool for skin burn assessment.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882W (22 February 2013); doi: 10.1117/12.2002927
Show Author Affiliations
Takeshi Yasui, Univ. of Tokushima (Japan)
Osaka Univ. (Japan)
Ryosuke Tanaka, Osaka Univ. (Japan)
Eiji Hase, Univ. of Tokushima (Japan)
Shu-ichiro Fukushima, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)


Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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