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Proceedings Paper

Two-Photon Fluorescence Stereomicroscopy with Bessel Beams
Author(s): Yanlong Yang; Ming Lei; Juanjuan Zheng; Runze Li; Shaohui Yan; Baoli Yao; Tong Ye
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Paper Abstract

Three dimensional distributions of cells can be usually acquired by optical sectioning methods, such as multiphoton excitation and confocal fluorescence laser scanning microscopy. Though the lateral scan rates can reach up to several kHz, the relatively slow axial scan comprises the speed of real-time imaging of a volume. Here we propose a three dimensional imaging method that uses Bessel beams as excitation in multiphoton fluorescence microscopy. The extended focus of the Bessel beam allows recording a volume of cells without scanning the depth. The depth information can be retrieved by recording a pair of parallax views of the same volume. We have demonstrated the stereoscope capability on a homebuilt two-photon fluorescence microscope.

Paper Details

Date Published: 22 February 2013
PDF: 5 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882K (22 February 2013); doi: 10.1117/12.2002850
Show Author Affiliations
Yanlong Yang, Xi'an Institute of Optics and Precision Mechanics (China)
Ming Lei, Xi'an Institute of Optics and Precision Mechanics (China)
Juanjuan Zheng, Xi'an Institute of Optics and Precision Mechanics (China)
Runze Li, Xi'an Institute of Optics and Precision Mechanics (China)
Shaohui Yan, Xi'an Institute of Optics and Precision Mechanics (China)
Baoli Yao, Xi'an Institute of Optics and Precision Mechanics (China)
Tong Ye, Xi'an Institute of Optics and Precision Mechanics (China)
The Univ. of Alabama at Birmingham (United States)


Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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