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Proceedings Paper

Two-photon excited endogenous fluorescence for label-free in vivo imaging ingestion of disease-causing bacteria by human leukocytes
Author(s): Yan Zeng; Bo Yan; Qiqi Sun; Seng Khoon Teh; Wei Zhang; Zilong Wen; Jianan Y. Qu
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Paper Abstract

Real time and in vivo monitoring leukocyte behavior provides unique information to understand the physiological and pathological process of infection. In this study, we demonstrate that two-photon excited reduced nicotinamide adenine dinucleotide (NADH) fluorescence provides imaging contrast to distinguish granulocyte and agranulocyte. By using spectral and time-resolved NADH fluorescence, we study the immune response of human neutrophils against bacterial infection (Escherichia coli). The two-photon excited NADH fluorescence images clearly review the morphological changes from resting neutrophils (round shape) to activated neutrophils (ruffle shape) during phagocytosis. The free-tobound NADH ratio of neutrophils decreases after ingesting disease-causing pathogen: Escherichia coli. This finding may provide a new optical tool to investigate inflammatory processes by using NADH fluorescence in vivo.

Paper Details

Date Published: 22 February 2013
PDF: 7 pages
Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881L (22 February 2013); doi: 10.1117/12.2002768
Show Author Affiliations
Yan Zeng, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Bo Yan, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Qiqi Sun, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Seng Khoon Teh, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Wei Zhang, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Zilong Wen, Hong Kong Univ. of Science and Technology (Hong Kong, China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong, China)


Published in SPIE Proceedings Vol. 8588:
Multiphoton Microscopy in the Biomedical Sciences XIII
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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