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Proceedings Paper

Non-invasive imaging of zebrafish with spinal deformities using optical coherence tomography: a preliminary study
Author(s): Liane Bernstein; Kathy Beaudette; Kessen Patten; Émilie Beaulieu-Ouellet; Mathias Strupler; Florina Moldovan; Caroline Boudoux
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Paper Abstract

A zebrafish model has recently been introduced to study various genetic mutations that could lead to spinal deformities such as scoliosis. However, current imaging techniques make it difficult to perform longitudinal studies of this condition in zebrafish, especially in the early stages of development. The goal of this project is to determine whether optical coherence tomography (OCT) is a viable non-invasive method to image zebrafish exhibiting spinal deformities. Images of both live and fixed malformed zebrafish (5 to 21 days postfertilization) as well as wild-type fish (5 to 29 days postfertilization) were acquired non-invasively using a commercial SD-OCT system, with a laser source centered at 930nm (λ=100nm), permitting axial and lateral resolutions of 7 and 8μm respectively. Using two-dimensional images and three-dimensional reconstructions, it was possible to identify the malformed notochord as well as deformities in other major organs at different stages of formation. Visualization of the notochord was facilitated with the development of a segmentation algorithm. OCT images were compared to HE histological sections and images obtained by calcein staining. Because of the possibility of performing longitudinal studies on a same fish and reducing image processing time as compared with staining techniques and histology, the use of OCT could facilitate phenotypic characterization in studying genetic factors leading to spinal deformities in zebrafish and could eventually contribute to the identification of the genetic causes of spinal deformities such as scoliosis.

Paper Details

Date Published: 8 March 2013
PDF: 6 pages
Proc. SPIE 8565, Photonic Therapeutics and Diagnostics IX, 85656H (8 March 2013); doi: 10.1117/12.2002142
Show Author Affiliations
Liane Bernstein, Ecole Polytechnique Montréal (Canada)
Kathy Beaudette, Ecole Polytechnique Montréal (Canada)
CHU Sainte-Justine and Univ. de Montréal (Canada)
Kessen Patten, CHU Sainte-Justine and Univ. de Montréal (Canada)
Émilie Beaulieu-Ouellet, Ecole Polytechnique Montréal (Canada)
Mathias Strupler, Ecole Polytechnique Montréal (Canada)
CHU Sainte-Justine and Univ. de Montréal (Canada)
Florina Moldovan, CHU Sainte-Justine and Univ. de Montréal (Canada)
Caroline Boudoux, Ecole Polytechnique Montréal (Canada)
CHU Sainte-Justine and Univ. de Montréal (Canada)


Published in SPIE Proceedings Vol. 8565:
Photonic Therapeutics and Diagnostics IX
Andreas Mandelis; Brian Jet-Fei Wong; Anita Mahadevan-Jansen; Henry Hirschberg; Hyun Wook Kang; Nikiforos Kollias; Melissa J. Suter; Kenton W. Gregory; Guillermo J. Tearney; Stephen Lam; Bernard Choi; Steen J. Madsen; Bodo E. Knudsen; E. Duco Jansen; Justus F. Ilgner; Haishan Zeng; Matthew Brenner; Laura Marcu, Editor(s)

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