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Proceedings Paper

Tumor-stem cells interactions by fluorescence imaging
Author(s): Aleksandra V. Meleshina; Elena I. Cherkasova; Ekaterina Sergeeva; Ilya V. Turchin; Ekaterina V. Kiseleva; Erdem B. Dashinimaev; Marina V. Shirmanova; Elena V. Zagaynova
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Paper Abstract

Recently, great deal of interest is investigation the function of the stem cells (SC) in tumors. In this study, we studied «recipient–tumor– fluorescent stem cells » system using the methods of in vivo imaging and laser scanning microscopy (LSM). We used adipose-derived adult stem (ADAS) cells of human lentiviral transfected with the gene of fluorescent protein Turbo FP635. ADAS cells were administrated into nude mice with transplanted tumor HeLa Kyoto (human cervical carcinoma) at different stages of tumor growth (0-8 days) intravenously or into tumor. In vivo imaging was performed on the experimental setup for epi – luminescence bioimaging (IAP RAS, Nizhny Novgorod). The results of the imaging showed localization of fluorophore tagged stem cells in the spleen on day 5-9 after injection. The sensitivity of the technique may be improved by spectral separation autofluorescence and fluorescence of stem cells. We compared the results of in vivo imaging and confocal laser scanning microscopy (LSM 510 META, Carl Zeiss, Germany). Internal organs of the animals and tumor tissue were investigated. It was shown that with i.v. injection of ADAS, bright fluorescent structures with spectral characteristics corresponding to TurboFP635 protein are locally accumulated in the marrow, lungs and tumors of animals. These findings indicate that ADAS cells integrate in the animal body with transplanted tumor and can be identified by fluorescence bioimaging techniques in vivo and ex vivo.

Paper Details

Date Published: 22 February 2013
PDF: 8 pages
Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871S (22 February 2013); doi: 10.1117/12.2001481
Show Author Affiliations
Aleksandra V. Meleshina, Nizhny Novgorod State Univ. (Russian Federation)
Elena I. Cherkasova, Nizhny Novgorod State Univ. (Russian Federation)
Ekaterina Sergeeva, Institute of Applied Physics (Russian Federation)
Ilya V. Turchin, Institute of Applied Physics (Russian Federation)
Ekaterina V. Kiseleva, Koltzov Institute of Developmental Biology (Russian Federation)
Erdem B. Dashinimaev, Koltzov Institute of Developmental Biology (Russian Federation)
Marina V. Shirmanova, Nizhny Novgorod State Medical Academy (Russian Federation)
Elena V. Zagaynova , Nizhny Novgorod State Medical Academy (Russian Federation)


Published in SPIE Proceedings Vol. 8587:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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