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Proceedings Paper

Multi-emitter colocalization in 3D stochastic optical reconstruction microscopy
Author(s): Yi Sun; Yang Pu; Mitchell Schaffler
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Paper Abstract

In this paper, we analyze the Cramer-Rao lower bound on the accuracy of 3-D emitter locations estimated from a STORM movie with a cylindrical lens. Numerical evaluation for randomly and uniformly distributed emitters indicates that as the emitter density in the image plane increases, the Cramer-Rao lower bounds on the average full-width half-maximum (FWHM) of location estimates in the lateral plane and axial direction increase exponentially fast; and the exponents can be numerically accurately estimated. The Cramer-Rao lower bounds are inversely proportional to the square root of mean number of photons of an emitter and monotonically decrease as the signal to noise ratio increases. The result reveals the insightful property of 3-D STORM movies and provides a benchmark for the achievable accuracy of location estimation algorithms. The developed algorithm with multiemitter colocalization can improve the temporal resolution by five folds compared with the single-emitter location estimator.

Paper Details

Date Published: 22 February 2013
PDF: 10 pages
Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900Y (22 February 2013); doi: 10.1117/12.2001157
Show Author Affiliations
Yi Sun, The City College, CUNY (United States)
Yang Pu, The City College, CUNY (United States)
Mitchell Schaffler, The City College, CUNY (United States)

Published in SPIE Proceedings Vol. 8590:
Single Molecule Spectroscopy and Superresolution Imaging VI
Jörg Enderlein; Ingo Gregor; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling, Editor(s)

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